His-Tag Mouse Monoclonal Antibody (Mix)
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FileTitle_Pre | BAB1169 |
FileTitle_Sub | His-Tag Mouse Monoclonal Antibody(Mix) |
Name | His-Tag Mouse Monoclonal Antibody (Mix) |
Cat.No. | BAB1169 |
Quantity | 100/50µL |
Applications | WB, IP, IF |
Cross Reacitivity | N/A |
Molecular Weight | N/A |
Isotype | IgG1 |
Storage Buffer | PBS, pH 7.4, containing 0.02% sodium azide and 50% Glycerol. |
Store at | -20°C. Do not aliquot the antibody. For research use only. |
Recommended ditultion | WB: 1:5,000-10,000, IP: 1:200, IF: 1:200 |
Specificity | A mixture mouse monoclonal antibody is prepared by immunizingsynthetic peptide.The antibody can recognize C-terminal, internal, and N-terminal His-tag fusionproteins. |
Background | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. |
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