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Brand	MultiSciences
CatNum	70-EK376
ProductName	Rat IgM ELISA Kit
CustomsName	Rat IgM ELISA Kit
Application	ELISA
Reactivity	Rat
Assay Type	Sandwich ELISA
Suitable Sample Type	serum, plasma, cell culture supernates
Format	96-well strip plate
Storage	4? (unopened) standard stored at -20?, others stored at 4? (opened)
Shipping Condition	4?
Sample Volume	20 µl (prediluted)
Sensitivity	0.17 ng/ml
Standard Curve Range	0.63 - 40 ng/ml
Spike Recovery Range	-
Mean Spike Recovery	-
CV of Intra plate	4.8 % - 7.7 %
CV of Inter plate	6.1 % - 7.1 %
Components	96-well polystyrene microplate coated with a monoclonal antibody against IgM Rat IgM Standard, lyophilized IgM Detect Antibody Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers
Describtion	This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IgM. The Rat IgM ELISA is for research use only. Not for use in therapeutic procedures. Immunoglobulin M (IgM) is a basic antibody that is produced by B cells. It is the first antibody to appear in response to initial exposure to an antigen. The spleen, where plasmablasts responsible for antibody production reside, is the major site of specific IgM production. IgM in normal serum is often found to bind to specific antigens, even in the absence of prior immunization. For this reason IgM has sometimes been called a "natural antibody". Demonstrating IgM antibodies in a patient's serum indicates recent infection, or in a neonate's serum indicates intrauterine infection.